CONSULT DOCUMENTATION FORM
https://labs.utsouthwestern.edu/sites/default/files/2022-06/dssr-project-request-form.docx
Data Science Shared Resource DataScience.SR@UTSouthwestern.edu Project Request Form PI Name(s): Email: Department: Brief description of project: Is this for a grant proposal?
abstract-hts-project-nih.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/abstract-hts-project-nih.pdf
Our objective for this proposal is to identify and chemically optimize compounds that enhance the secretion of retained mutant UMOD protein as a new therapeutic modality for treating ADTKD-UMOD.
abstract-dod-grant.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/abstract-dod-grant.pdf
Our research showed that urinary proteins Mucin-1 (MUC1) and Uromodulin (UMOD) upregulate TRPM6 from the luminal side of the tubule. In Umod -/- mice, we found renal Mg 2+ wasting and, in contrast to wild-type (WT) animals, impaired glucose tolerance when fed a low Mg 2+ diet.
ut-irb-11979-registry-trials-flier-rev4-3-30-1.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/ut-irb-11979-registry-trials-flier-rev4-3-30-1.pdf
Volunteers Needed For Clinical Research at UT Southwestern. We are conducting studies in obesity, heart disease risk, and type 2 diabetes risk in adults. Please contact us at (214) 648-9014. Compensation available for some studies.
Microsoft Word - TOPAZ-Relion-v2.docx
https://labs.utsouthwestern.edu/sites/default/files/2022-07/TOPAZ-Relion-v2.pdf
In the “Input” tab, give Relion the script for training TOPAZ, as well as the micrographs you want TOPAZ to be trained on and the set of particles for training. d.
FACLR_Human_Fibroblast_SOP
https://labs.utsouthwestern.edu/sites/default/files/2022-07/FACLR_Human_Fibroblast_SOP.pdf
1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050
ut-irb-no-11607-896-body-fat-clinical-trial-022811-final.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/ut-irb-no-11607-896-body-fat-clinical-trial-022811-final.pdf
M KT 8 96 _0 3 Qualified participants must be: • Between age 18–70 years • South Asian or European Americans • Male and Female • Able to attend two visits • Persons taking medications for diabetes, heart disease or chronic health issues may not qualify For more information 214.648.9014
SBL_VitrobotProtocol_0.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/SBL_VitrobotProtocol_0.pdf
1 SBL Vitrobot Protocol (based on the Hibb’s lab protocol, modified by Xiaochen and James) Materials Required (your own supply or from SBL): - Grids. - Vitrobot Tweezer Assembly.
SBL Fee Sheet_2022.xlsx
https://labs.utsouthwestern.edu/sites/default/files/2022-07/SBLfee-sheet_2022.pdf
$31.00 $46.50 24‐well crystallization tray (VDX) 15‐well crystallization tray (Nextal/Qiagen) 24‐well crystallization tray (Cryschem) Safematic compact coating unit training Talos Arctica usage, unassisted, hourly Titan Krios usage, unassisted, hourly Manuscript Preparation Synchrotron Data Collection
PixelSize_DoseRate.pdf
https://labs.utsouthwestern.edu/sites/default/files/2022-07/PixelSize_DoseRate.pdf
Pixel Size and Dose information 1 Set up parameters (ss, C2/illumination area) precisely for data collection: Find a target/hole -> On Gatan (K3) PC -> DM -> take an image Image Properties -> Pixel Size Dose information Find an empty area or undock grid (use vacuum) -